Giemsa staining for cysts and trophozoites of Pneumocystis carinii.

Giemsa staining for cysts and trophozoites of Pneumocystis carinii.

Although Giemsa staining has been routinely used for the detection of trophozoites and intracystic bodies in smears of bronchoalveolar lavage fluid (BAL) from patients with Pneumocystis carinii pneumonia, it does not normally stain the cyst wall. For detection of the cysts other stains such as toluidine Blue 'O' and methenamine silver must be used as well. Sulphation of smears before staining w...

Why have Pneumocystis carinii trophozoites been ignored?

مقایسه لام های تماسی و برشهای بافتی با استفاده از روشهای رنگ آمیزی مختلف در تشخیص عفونت پنوموسیستیس کارینی

Background & Aims: Pneumocystis carinii is an opportunist extracellular microorganism. The body can defend itself against it in natural conditions. But it can cause an infection in immunodificient conditions such as HIV, Hodgkins and other immunosuppressed diseases. The aim of this study was to apply a comparison of different staining methods such as Methenamine silver, Giemsa, Gram - Weigert, ...

Pneumocystis carinii.

Although Giemsa staining has been routinely used for the detection of trophozoites and intracystic bodies in smears of bronchoalveolar lavage fluid (BAL) from patients with Pneumocystis cariniipneumonia, it does not normally stain the cyst wall. For detection ofthe cysts other stains such as toluidine Blue 'O' and methenamine silver must be used as well. Sulphation of smears before staining wit...

Growth of Pneumocystis Carinii in Axenic Culture

SUMMARY In this research, which was done for two years, we investigated various liquid and solid media without feeder ceells, for the ability to support growth of P. carinii. Data obtained from counting the organisms nuclei showed that, addition of Cyclo­heximide, penicillin, & streptomycin to neopeptone­N-Acetyle Glucoseamine (N.P.G. medium) can provides growth requirements of organism in its...